Biotechnology Applications Hammer Studies

What is PCR?

PCR stands for Polymerase Chain Reaction. It amplifies one segment of DNA and makes between one copy and one billion copies of that segment of DNA. This makes many copies of the same DNA segment to use elsewhere. This technique is used is used in molecular biology.

How does PCR work?

PCR works by putting a DNA strand in extreme heat in order to denature it. Then it splits into two seperate single stranded DNA segments. Then Taq polymerase synthesizes and builds a new single stranded DNA to pair with the other part of the DNA that was split up. This makes 2 new strands of DNA.

Provide one possible real world application for PCR.

One way that PCR can be used in the real world is to find out if there is a specific virus in your body.

What is gel electrophoresis?

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size.

How does gel electrophoresis work?

In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

Provide one possible real world application for gel electrophoresis.

You can use gel electrophoresis to get DNA fingerprints for forensics.

What is DNA sequencing?

DNA sequencing is used to find the order of of nucleotides in DNA molecule. It includes any method or technology that is used to determine the order of the four bases of adenine, guanine, cytosine, and thymine

Provide one possible real world application for DNA sequencing.

One real world application for DNA sequencing is to determine help duplicate certain segments of DNA.

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