Gel Electrophoresis By Caleb Lytle, Colten Crager, Thomas Woeste, Dawson Spraggins, and Adam wier

Procedures

  1. Measure 50mL of TAE buffer and pour into beaker
  2. Measure .8g of agarose and pour into beaker
  3. Heat beaker at 265 degrees Celsius and stir until no particulate can be seen at the bottom
  4. Pour mixture into the gel trays.
  5. Insert a comb into the tray.
  6. Allow time to cool.
  7. Place cooled gel in gel electrophoresis chamber.
  8. Pour TAE buffer to cover gel
  9. Transfer DNA samples into respective wells
  10. Turn on Gel Electrophoresis chamber.
  11. Remove gel from chamber and coat the gel in stain.

Results

Well

(left right wells away 1-5)

1- child number 2

2- child number 1

3- wild type

4- mutated

5- no DNA

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