Electrophoresis and Simulated Genetic Screen By: Callie Pettigrew, Leah Collins, Maddie Yeagle, Carmen Downing, and Chase Stanback

Procedures:

  1. First we make the gel by: Add .5 grams of agarose at 1 % to 50 mL to the buffer, then mix and heat on the heat plate until the solution is clear.
  2. Then remove from heat and pour into the mold.
  3. Allow the gel to sit over night in the refrigerator.
  4. Then place the gel in the electrophoresis chamber and label the DNA vials one to five. One is no DNA, two is child #2, three is wild-type, four is mutated, five is child #1.
  5. Submerge the gel with enough buffer to cover the wells.
  6. Proceed to carefully add the DNA into the right well.
  7. Hook up the chamber to the electrical box.
  8. Observe and record data accordingly.
  9. Stain the dye.
  10. Leave in the refrigerator over night.
  11. Examine the gel and record the data.
  12. Have a nice day.

Lab Supplies

  • Graduated Cylinder
  • Beaker
  • Scale
  • Disposable Paper for agarose
  • Hot stir plate with magnent
  • 0.5 grams of agarose
  • 50 mL of buffer
  • Mold with well maker
  • Refrigerator
  • Electric Chambor
  • Blue dye
  • Needle nose Pipet
  • 5 different types of DNA
  • Holder for the gel to sit overnight

Summary

While we only observed minimal amounts of data, the experiment still illustrated the separation of DNA fragments. Out of the five DNA samples we tested in the gel, only one of the DNA wells showed results. The fragments were lines of DNA that were separated from each other based on their base sizes from the different enzyme cuttings. The second well, (Child #2), produced two fragments of DNA which were 1 mm apart, and the first fragment was 2.5 mm from the initial well. This experiment was partially a success because this was the first time performing the experiment and we still got some results.

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