Lactase Lab by Nicole, Angela, and Jessie

Materials

Lactase Tablet

15 ml of skim milk

Water

Sucrose

100 ml Graduated Cylinder

10 ml graduated cylinder

3 400 ml Beakers

5 test tubes

Test tube reach

Marking pencil

Timer

Hot plate with a Pyrex test tube

Glucose test strips

Stirring Rod

PREPARATION AND PROCEDURE

Make the Enzyme Solution out of a 1 tablet of lactase tablet and 200 ml of water.

Make the Sucrose Solution by adding 5 grams of sugar to 100 ml of water.

Make the Denatured Enzyme Solution by putting 20 ml of the Enzyme solution into a tube and placeint it into a beaker with 200 ml of water then boil for 30 minutes then cool to room temperature.

Gather Materials and Label 5 test tubes.

  1. In test tube A, add 2 ml of skim milk and 1 ml f the enzyme solution. Time for 2 minutes. Test for glucose and mark + if it is there
  2. In test tube B, add 2 ml of skim milk and 1 ml of water. Time for 2 minutes. Test for glucose and mark + if it is there
  3. In test tube C, add 2 ml of skim milk and 1 ml of denatured enzyme solution.
  4. In test tube D add 2 ml of the sucrose solution and 1 ml of enzyme solution. Time for 2 minutes. Test for glucose and mark + if it is there
  5. In test tube E add 2 ml of the sucrose solution and 1 ml of water. Time for 2 minutes. Test for glucose and mark + if it is there

Clean up your station. All solutions used in this experiment can be paired down the sink.

RESULTS

ANALYSIS

Sucrose has a different shape than lactose does, therefore the enzyme lactase did not react with the sucrose to break it down into glucose and fructose.

When the enzyme was boiled it was denatured so it did not properly function, as in break down the lactose into glucose and galactose.

Ways to affect the enzyme include raising the temperature and lowering the pH level. Lactase however is supposed to work in the stomach. Lowering the pH of the enzyme solution would not affect the enzyme because the stomach already has a low pH and Lactase is meant to work in the acidic area.

Lactase is speeding up hydrolysis because it speeds up the breaking apart of chemical bonds. With this lab, we were breaking apart lactose to get glucose and galactose.

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