Investigation
TLC (Thin Layer Chromatography) and UV / VIS spectroscopy were used to determine the main pigments presents in the spinach leaves and their concentration. Here is the method we used.
Collecting data and data analysis
We compared and analyzed the obtained data and interpreted the results. Here are TLC's result.
Our TLC analyses says that in spinach leaves you find, moving from the origin to the front: A Chlorophyllid (light green); Xantophyll (Yellow); B Chlorophyll (Yellow-green); A Chlorophyll (Blue-green); Phaeophytins (Grey); Carotenes (Yellow)
Collecting data and data analysis
Here are UV / VIS spectroscopy's results.
The absorption spectrum of extract showed that the most intense absorption values are between 440 nm and 500 nm (a range that best represents carotenoids)
On the other hand, less intense absorption values were observed between 650 nm and 700 nm (the interval that best represents chl a)
Credits:
Created with images by Val Vesa - "Stay the Course" • Nikola Briede - "untitled image" • pandu ior - "Triangle Life" • ejaugsburg - "environmental protection nature conservation ecology" • pixel2013 - "trees forest forest path" • ponce_photography - "spinach healthy green"