Electrophoresis Lab Jake, Emma, Josiah, Nick, Lynaia, Dylan

We had 1.2 percent agarose(weighed 6 grams)We also mixed in 50- milligrams of TAE buffer .Next we heated the mixture on a hot plate and used a magnetic stirrer to mix the solution for about 7 minutes at 164 degrees Celsius. After waiting three minutes we filled a tray with the mixture to form a thin cube. Next we took the gel which was now in a solid gel form out of the case and we removed the comb from the gel leaving air pockets for the DNA . Next we inserted four different types named(Child1, Child2, Wild type, and mutated) of DNA into pocket of the gel using a pipet. Next we filled the electrophoresis chamber with buffer and put the gel in the chamber with the end of the gel that has wells toward the negative end. After we let the electrophoresis chamber run until the bromphenol blue band was 1cm from the well; we stained the gel with CarolinaBLU for 20 minutes, waited 45 minutes after to de-stain it, and then riced it with deionized water. Next we measured the rings distance from the wells in millimeters using a ruler and then compared there lengths to find similarities.

At this step we are filling the case with the gel so that we can insert the DNA Later
Here we are measuring and pouring the agarose into the flask
This is the result of the gel after we inserted the DNA and put it through the gel electrophoresis chamber
This is the result of the gel after we stained the gel with CarolinaBLU

Result

Child 1 had 3 bands Distance from well: 80mm

Child 2 had 3 bands Distance from well: 80mm, 110mm , 120mm

The Wild type DNA had 2 band Distances from well: 80mm

The Mutated DNA had two bands Distances from well: 80mm

Conclusion: Child 1 and 2 are related because they have similar band distances from the wells at 120mm. Child 2 , mutated and wild type have a similar distance from the well(80mm) meaning that they are also related. Also what we found when comparing our result to those of another group that had a different concentration of agarose, we concluded that it is better to have a higher concentration of agarose to do this expirament.

Created By
Josiah Charley
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