Polymerase Chain Reaction (PCR) & Gel Electrophoresis
Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size.
How Is It Done?
In gel electrophoresis, the DNA strands that are to be separated are pushed by an electrical field through a gel that contains small pores.
Are Polymerase Chain Reactions (PCR) & Gel Electrophoresis Used Today?
Yes, they are used in criminal investigation cases all the time, and I'm many other scenarios.
The only ethical issues involved with this, would be with the dangers of dealing with electricity, and although the harm it can cause to someone is minimal, it could still be dangerous to deal with.
Pros & Cons...
Pros: The pore size of acrylamide are uniform.
It is inert and not react with sample since there is no charge associated with it.
Very high resolving power.
Can increase or decrease molecular sieving by manipulate the pore sizes.
Different buffers can be used to manipulate resolution and run time.
Chemically synthethic gel can give more reproducible results.
Promote better separations at higher field strengths due to efficient heat dissipation of thin gels (gel-electrophoresis.wixsite.com).
Cons: Acrylamide and TEMED are neurotoxic compounds. Gloves should be wear when handling solutions of acrylamide and TEMED.
Longer wait time for gel to set.
More care needed when pouring the gel to ensure gas bubbles are removed (gel-electrophoresis.wixsite.com).
How is Technology Used in Polymerase Chain Reactions (PCR) & Gel Electrophoresis?
Generally, the gel consist pf acrylamide, bisacrylamide, the optional denaturant (SDS) and a buffer with an adjusted pH, Electricity, stabilizer (such as ammonium per sulfate and TEMED), gel caster, filters, syringes, and many other scientific lab Technology.
Did You Know?
There are two different ways to read the DNA fragments in the gel, one is staining the gel and looking at the DNA fragments that have absorbed the color, and the other way is to look under an ultraviolet lamp (a black light) and they will appear.