BIOGRAPHY

• Abdul Hamood received his B.S. degree in veterinary medicine and surgery from the University of Baghdad, Iraq, in 1977. He received his M.S. degree in veterinary medicine from the University of Missouri, Columbia, USA, in 1984 and his Ph.D. degree in medical microbiology from the School of Medicine at the same institution in 1985.
• He was a Research Fellow at the University of Rochester, Rochester, New York, NY, USA, from 1986 to 1990. In 1991, he was pointed as an Assistant Professor at the Department of Microbiology and Immunology at Texas Tech University Health Sciences Center, Lubbock, Texas, USA. Currently, he serves as a Professor at the same department. His research is focused on the pathogenesis of Pseudomonas aeruginosa infections.

RESEARCH

Our research interests involve the regulation of Pseudomonas aeruginosa, virulence genes, and the inhibition of biofilm development by bacterial pathogens.

A. Regulation of P. aeruginosa virulence genes

1. Global regulation of P. aeruginosa genes by ptxR:

• P. aeruginosa is an opportunistic pathogen that causes serious infections in patients with cystic fibrosis (CF), severe burns, and immunosuppression, including patients with HIV infections and neonates.
• P. aeruginosa produces several cell-associated and extracellular virulence factors. Cell-associated factors include alginate, pili, and lipopolysaccharide, while extracellular factors include exotoxin A, exoenzyme S, elastase, phospholipase C, and the siderophores. Exotoxin A is an ADP-ribosyl tranferase enzyme that interferes with the protein synthesis process, and causes cell death. We have isolated a P. aeruginosa gene, ptxR, which enhances toxA at the transcription level.
• Further studies confirmed that PtxR is a global regulator that regulates the expression of several of P. aeruginosa virulence genes. PtxR regulates the expression of many of these genes through the density dependent cell-to-cell communication, or quorum sensing (QS) systems. Transcriptional studies revealed that PtxR regulates the expression of all components of the QS systems. The effect of ptxR on the QS system is unique in that it enhances the expression of certain genes but represses the expression of others (See Image above). We are currently investigating the specific mechanism(s) through which PtxR regulates these genes.

2. Serum albumin alters the expression of Pseudomonas aeruginosa iron controlled genes:

• The production of P. aeruginosa virulence factors systemically and certain infection sites is likely to be influenced by serum. During systemic infections and within infected wounds, P. aeruginosa is exposed to serum or components of serum. We analyzed the effect of serum on the expression of P. aeruginosa genes using microarray experiments. Serum influenced global gene expression in P. aeruginosa at the early exponential phase of growth. Serum enhanced the expression of 154 genes, many of which are iron-controlled. The mechanism of enhancement appears to be independent of iron and the ferric uptake regulator Fur. Serum also influences the expression of different QS genes. Our goal is to define the complicated network through which serum regulates the expression of these genes.

B. Inhibiting the development of bacterial biofilms on medical devices

• Within different sites (including burn wounds), infecting microorganisms often exist in specific structures termed biofilms. Mature biofilms are usually described as mushroom-shaped multicellular structures with channels that presumably function in delivering nutrients and removing wastes. Within the biofilm, the microorganisms are surrounded by a glycocalyx composed of a combination of an extracellular matrix (EPS) that is produced by the microorganisms and the host surrounding tissues and protect the bacteria from the effect of different antibiotics. Due to the alarming rate at which antibiotic resistant strains emerge, we are testing alterative novel approaches to prevent the development of bacterial biofilms and/or eliminating them.
• Previous studies have shown that selenium (Se) bound to a solid matrix retains its ability to catalyze the formation of superoxide radicals (O2•¯). These superoxide radicals interfere with bacterial attachment to solid surfaces.
• In collaboration with Dr. Ted Reid (department of Ophthalmology, TTUHSC), Spire Corporation, Bedford, MA, and Selenium Ltd., Austin, TX, we investigated the effectiveness of selenocyanatodiacetic acid (SCAA) in inhibiting the development of S. aureus biofilms on hemodialysis catheters. The inner and outer surfaces of hemodialysis catheters were coated with SCAA and the efficacy of coated catheters in inhibiting S. aureus biofilms in vitro and in vivo was examined. S. aureus developed biofilms on both sides of uncoated catheters but not on SAA-coated ones. Similarly, using the modified murine model of chronic biofilm infection, we confirmed that S. aureus failed to develop a biofilm on either side of the SCAA-coated catheters in vivo (See Image 2). Currently, we are investigating the effectiveness of Selenium coating in inhibiting the development of bacterial biofilms on other medical devices.

PEOPLE

PUBLICATIONS

• Cassandra Kruczek, Kameswara Rao Kottapalli, Sharmila Dissanaike, Nyaradzo Dzvova, John A. Griswold, Jane A. Colmer-Hamood, Abdul N. Hamood. Major Transcriptome Changes Accompany the Growth of Pseudomonas aeruginosa in Blood from Patients with Severe Thermal Injuries. PLoS One. 2016; 11(3): e0149229. Published online 2016 Mar 2. doi: 10.1371/journal.pone.0149229. PMCID: PMC4774932
• Banerjee D, Tran PL, Colmer-Hamood JA, Wang JC, Myntti M, Cordero J, Hamood AN. The antimicrobial agent, Next-Science, inhibits the development of Staphylococcus aureus and Pseudomonas aeruginosa biofilms on tympanostomy tubes. Int J Pediatr Otorhinolaryngol. 2015 Nov;79(11):1909-14. doi: 10.1016/j.ijporl.2015.09.003. Epub 2015 Sep 7. PubMed PMID: 26388185.
• Elmasri WA, Yang T, Tran P, Hegazy ME, Hamood AN, Mechref Y, Paré PW. Teucrium polium phenylethanol and iridoid glycoside characterization and flavonoid inhibition of biofilm-forming Staphylococcus aureus. J Nat Prod. 2015 Jan 23;78(1):2-9. doi: 10.1021/np5004092. Epub 2014 Dec 19. PubMed PMID: 25524452.
• Tran PL, Hamood AN, de Souza A, Schultz G, Liesenfeld B, Mehta D, Reid TW. A study on the ability of quaternary ammonium groups attached to a polyurethane foam wound dressing to inhibit bacterial attachment and biofilm formation. Wound Repair Regen. 2015 Jan-Feb;23(1):74-81. doi: 10.1111/wrr.12244. Epub 2015 Feb 13. PubMed PMID: 25469865.
• Wang JC, Hamood AN, Saadeh C, Cunningham MJ, Yim MT, Cordero J. Strategies to prevent biofilm-based tympanostomy tube infections. Int J Pediatr Otorhinolaryngol. 2014 Sep;78(9):1433-8. doi: 10.1016/j.ijporl.2014.05.025. Epub 2014 Jun 30. Review. PubMed PMID: 25060938.
• Elmasri WA, Hegazy ME, Aziz M, Koksal E, Amor W, Mechref Y, Hamood AN, Cordes DB, Paré PW. Biofilm blocking sesquiterpenes from Teucrium polium. Phytochemistry. 2014 Jul;103:107-13. doi: 10.1016/j.phytochem.2014.03.029. Epub 2014 Apr 12. PubMed PMID: 24735824.
• Miller KG, Tran PL, Haley CL, Kruzek C, Colmer-Hamood JA, Myntti M, Hamood AN. Next science wound gel technology, a novel agent that inhibits biofilm development by gram-positive and gram-negative wound pathogens. Antimicrob Agents Chemother. 2014 Jun;58(6):3060-72. doi: 10.1128/AAC.00108-14. Epub 2014 Mar 17. PubMed PMID: 24637684; PubMed Central PMCID: PMC4068445.
• Haley CL, Kruczek C, Qaisar U, Colmer-Hamood JA, Hamood AN. Mucin inhibits Pseudomonas aeruginosa biofilm formation by significantly enhancing twitching motility. Can J Microbiol. 2014 Mar;60(3):155-66. doi: 10.1139/cjm-2013-0570. Epub 2014 Jan 24. PubMed PMID: 24588389; PubMed Central PMCID: PMC4199588.
• Kruczek C, Qaisar U, Colmer-Hamood JA, Hamood AN. Serum influences the expression of Pseudomonas aeruginosa quorum-sensing genes and QS-controlled virulence genes during early and late stages of growth. Microbiologyopen. 2014 Feb;3(1):64-79. PubMed PMID: 24436158; PubMed Central PMCID: PMC3937730.
• Balyimez A, Colmer-Hamood JA, San Francisco M, Hamood AN. Characterization of the Pseudomonas aeruginosa metalloendopeptidase, Mep72, a member of the Vfr regulon. BMC Microbiol. 2013 Nov 27;13:269. doi: 10.1186/1471-2180-13-269. PubMed PMID: 24279383; PubMed Central PMCID: PMC4222646.
• Wang JC, Tran PL, Hanes R, Cordero J, Marchbanks J, Reid TW, Colmer-Hamood JA, Hamood AN. Inhibition of otopathogenic biofilms by organoselenium-coated tympanostomy tubes. JAMA Otolaryngol Head Neck Surg. 2013 Oct;139(10):1009-16. PubMed PMID: 24030785.
• Qaisar U, Luo L, Haley CL, Brady SF, Carty NL, Colmer-Hamood JA, Hamood AN. The pvc operon regulates the expression of the Pseudomonas aeruginosa fimbrial chaperone/usher pathway (cup) genes. PLoS One. 2013 Apr 30;8(4):e62735. doi: 10.1371/journal.pone.0062735. Print 2013. PubMed PMID: 23646138; PubMed Central PMCID: PMC3639982.
• Haley CL, Colmer-Hamood JA, Hamood AN. Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium. BMC Microbiol. 2012 Aug 18;12:181. doi: 10.1186/1471-2180-12-181. PubMed PMID: 22900764; PubMed Central PMCID: PMC3494610.
• Nidadavolu P, Amor W, Tran PL, Dertien J, Colmer-Hamood JA, Hamood AN. Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds. J Med Microbiol. 2012 May;61(Pt 5):662-71. doi: 10.1099/jmm.0.038638-0. Epub 2012 Feb 2. PubMed PMID: 22301617.
• Tran PL, Lowry N, Campbell T, Reid TW, Webster DR, Tobin E, Aslani A, Mosley T, Dertien J, Colmer-Hamood JA, Hamood AN. An organoselenium compound inhibits Staphylococcus aureus biofilms on hemodialysis catheters in vivo. Antimicrob Agents Chemother. 2012 Feb;56(2):972-8. doi: 10.1128/AAC.05680-11. Epub 2011 Nov 28. PubMed PMID: 22123688; PubMed Central PMCID: PMC3264236.
• Kruczek C, Wachtel M, Alabady MS, Payton PR, Colmer-Hamood JA, Hamood AN. Serum albumin alters the expression of iron-controlled genes in Pseudomonas aeruginosa. Microbiology. 2012 Feb;158(Pt 2):353-67. doi: 10.1099/mic.0.053371-0. Epub 2011 Nov 3. PubMed PMID: 22053004; PubMed Central PMCID: PMC3352286.
• Hammond AA, Miller KG, Kruczek CJ, Dertien J, Colmer-Hamood JA, Griswold JA, Horswill AR, Hamood AN. An in vitro biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates. Burns. 2011 Mar;37(2):312-21. doi: 10.1016/j.burns.2010.09.017. Epub 2010 Dec 3. PubMed PMID: 21130579; PubMed Central PMCID: PMC3034806.
• Li W, Lyte M, Freestone PP, Ajmal A, Colmer-Hamood JA, Hamood AN. Norepinephrine represses the expression of toxA and the siderophore genes in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2009 Oct;299(1):100-9. doi: 10.1111/j.1574-6968.2009.01739.x. Epub 2009 Jul 27. PubMed PMID: 19686346; PubMed Central PMCID: PMC2889019.
• Hammond A, Dertien J, Colmer-Hamood JA, Griswold JA, Hamood AN. Serum inhibits P. aeruginosa biofilm formation on plastic surfaces and intravenous catheters. J Surg Res. 2010 Apr;159(2):735-46. doi: 10.1016/j.jss.2008.09.003. Epub 2008 Oct 7. PubMed PMID: 19482317.
• Davinic M, Carty NL, Colmer-Hamood JA, San Francisco M, Hamood AN. Role of Vfr in regulating exotoxin A production by Pseudomonas aeruginosa. Microbiology. 2009 Jul;155(Pt 7):2265-73. doi: 10.1099/mic.0.028373-0. Epub 2009 Apr 23. PubMed PMID: 19389782; PubMed Central PMCID: PMC2888118.
• Tran PL, Hammond AA, Mosley T, Cortez J, Gray T, Colmer-Hamood JA, Shashtri M, Spallholz JE, Hamood AN, Reid TW. Organoselenium coating on cellulose inhibits the formation of biofilms by Pseudomonas aeruginosa and Staphylococcus aureus. Appl Environ Microbiol. 2009 Jun;75(11):3586-92. doi: 10.1128/AEM.02683-08. Epub 2009 Apr 3. PubMed PMID: 19346348; PubMed Central PMCID: PMC2687307.
• Ferrell E, Carty NL, Colmer-Hamood JA, Hamood AN, West SE. Regulation of Pseudomonas aeruginosa ptxR by Vfr. Microbiology. 2008 Feb;154(Pt 2):431-9. doi: 10.1099/mic.0.2007/011577-0. PubMed PMID: 18227247.
• Gaines JM, Carty NL, Tiburzi F, Davinic M, Visca P, Colmer-Hamood JA, Hamood AN. Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen. Microbiology. 2007 Dec;153(Pt 12):4219-33. PubMed PMID: 18048935.
• Alatoom AA, Aburto R, Hamood AN, Colmer-Hamood JA. VceR negatively regulates the vceCAB MDR efflux operon and positively regulates its own synthesis in Vibrio cholerae 569B. Can J Microbiol. 2007 Jul;53(7):888-900. PubMed PMID: 17898844.
• Schaber JA, Triffo WJ, Suh SJ, Oliver JW, Hastert MC, Griswold JA, Auer M, Hamood AN, Rumbaugh KP. Pseudomonas aeruginosa forms biofilms in acute infection independent of cell-to-cell signaling. Infect Immun. 2007 Aug;75(8):3715-21. Epub 2007 Jun 11. PubMed PMID: 17562773; PubMed Central PMCID: PMC1952004.
• Schaber JA, Hammond A, Carty NL, Williams SC, Colmer-Hamood JA, Burrowes BH, Dhevan V, Griswold JA, Hamood AN. Diversity of biofilms produced by quorum-sensing-deficient clinical isolates of Pseudomonas aeruginosa. J Med Microbiol. 2007 Jun;56(Pt 6):738-48. PubMed PMID: 17510257.
• Carty NL, Layland N, Colmer-Hamood JA, Calfee MW, Pesci EC, Hamood AN. PtxR modulates the expression of QS-controlled virulence factors in the Pseudomonas aeruginosa strain PAO1. Mol Microbiol. 2006 Aug;61(3):782-94. Epub 2006 Jun 27. PubMed PMID: 16803594.
• Colmer-Hamood JA, Aramaki H, Gaines JM, Hamood AN. Transcriptional analysis of the Pseudomonas aeruginosa toxA regulatory gene ptxR. Can J Microbiol. 2006 Apr;52(4):343-56. PubMed PMID: 16699585.
• Westfall LW, Carty NL, Layland N, Kuan P, Colmer-Hamood JA, Hamood AN. mvaT mutation modifies the expression of the Pseudomonas aeruginosa multidrug efflux operon mexEF-oprN. FEMS Microbiol Lett. 2006 Feb;255(2):247-54. PubMed PMID: 16448502.
• Haynes A 3rd, Ruda F, Oliver J, Hamood AN, Griswold JA, Park PW, Rumbaugh KP. Syndecan 1 shedding contributes to Pseudomonas aeruginosa sepsis. Infect Immun. 2005 Dec;73(12):7914-21. PubMed PMID: 16299282; PubMed Central PMCID: PMC1307082.
• Woolley RC, Vediyappan G, Anderson M, Lackey M, Ramasubramanian B, Jiangping B, Borisova T, Colmer JA, Hamood AN, McVay CS, Fralick JA. Characterization of the Vibrio cholerae vceCAB multiple-drug resistance efflux operon in Escherichia coli. J Bacteriol. 2005 Aug;187(15):5500-3. PubMed PMID: 16030246; PubMed Central PMCID: PMC1196037.
• Gaines JM, Carty NL, Colmer-Hamood JA, Hamood AN. Effect of static growth and different levels of environmental oxygen on toxA and ptxR expression in the Pseudomonas aeruginosa strain PAO1. Microbiology. 2005 Jul;151(Pt 7):2263-75. PubMed PMID: 16000716.
• Haynes A 3rd, Rumbaugh KP, Park PW, Hamood AN, Griswold JA. Protamine sulfate reduces the susceptibility of thermally injured mice to Pseudomonas aeruginosa infection. J Surg Res. 2005 Jan;123(1):109-17. PubMed PMID: 15652958.
• Westfall LW, Luna AM, San Francisco M, Diggle SP, Worrall KE, Williams P, Cámara M, Hamood AN. The Pseudomonas aeruginosa global regulator MvaT specifically binds to the ptxS upstream region and enhances ptxS expression. Microbiology. 2004 Nov;150(Pt 11):3797-806. PubMed PMID: 15528665.
• Schaber JA, Carty NL, McDonald NA, Graham ED, Cheluvappa R, Griswold JA, Hamood AN. Analysis of quorum sensing-deficient clinical isolates of Pseudomonas aeruginosa. J Med Microbiol. 2004 Sep;53(Pt 9):841-53. PubMed PMID: 15314190.
• Williams SC, Patterson EK, Carty NL, Griswold JA, Hamood AN, Rumbaugh KP. Pseudomonas aeruginosa autoinducer enters and functions in mammalian cells. J Bacteriol. 2004 Apr;186(8):2281-7. PubMed PMID: 15060029; PubMed Central PMCID: PMC412166.
• Rumbaugh KP, Hamood AN, Griswold JA. Cytokine induction by the P. aeruginosa quorum sensing system during thermal injury. J Surg Res. 2004 Jan;116(1):137-44. PubMed PMID: 14732360.
• Carty NL, Rumbaugh KP, Hamood AN. Regulation of toxA by PtxR in Pseudomonas aeruginosa PA103. Can J Microbiol. 2003 Jul;49(7):450-64. PubMed PMID: 14569286.
• Fogle MR, Griswold JA, Oliver JW, Hamood AN. Anti-ETA IgG neutralizes the effects of Pseudomonas aeruginosa exotoxin A. J Surg Res. 2002 Jul;106(1):86-98. PubMed PMID: 12127813.
• Rumbaugh KP, Colmer JA, Griswold JA, Hamood AN. The effects of infection of thermal injury by Pseudomonas aeruginosa PAO1 on the murine cytokine response. Cytokine. 2001 Nov 21;16(4):160-8. PubMed PMID: 11792126.
• Colmer JA, Hamood AN. Molecular analysis of the Pseudomonas aeruginosa regulatory genes ptxR and ptxS. Can J Microbiol. 2001 Sep;47(9):820-8. PubMed PMID: 11683464.
• Rumbaugh KP, Griswold JA, Hamood AN. The role of quorum sensing in the in vivo virulence of Pseudomonas aeruginosa. Microbes Infect. 2000 Nov;2(14):1721-31. Review. PubMed PMID: 11137045.
• Rashid MH, Rumbaugh K, Passador L, Davies DG, Hamood AN, Iglewski BH, Kornberg A. Polyphosphate kinase is essential for biofilm development, quorum sensing, and virulence of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9636-41. PubMed PMID: 10931957; PubMed Central PMCID: PMC16917.
• Swanson BL, Hager P, Phibbs P Jr, Ochsner U, Vasil ML, Hamood AN. Characterization of the 2-ketogluconate utilization operon in Pseudomonas aeruginosa PAO1. Mol Microbiol. 2000 Aug;37(3):561-73. PubMed PMID: 10931350.
• Swanson BL, Hamood AN. Autoregulation of the Pseudomonas aeruginosa protein PtxS occurs through a specific operator site within the ptxS upstream region. J Bacteriol. 2000 Aug;182(15):4366-71. PubMed PMID: 10894751; PubMed Central PMCID: PMC101966.
• Colmer JA, Hamood AN. Expression of ptxR and its effect on toxA and regA expression during the growth cycle of Pseudomonas aeruginosa strain PAO1. Can J Microbiol. 1999 Dec;45(12):1008-16. PubMed PMID: 10696480.
• Rumbaugh KP, Griswold JA, Hamood AN. Pseudomonas aeruginosa strains obtained from patients with tracheal, urinary tract and wound infection: variations in virulence factors and virulence genes. J Hosp Infect. 1999 Nov;43(3):211-8. PubMed PMID: 10582188.
• Rumbaugh KP, Griswold JA, Iglewski BH, Hamood AN. Contribution of quorum sensing to the virulence of Pseudomonas aeruginosa in burn wound infections. Infect Immun. 1999 Nov;67(11):5854-62. PubMed PMID: 10531240; PubMed Central PMCID: PMC96966.
• Swanson BL, Colmer JA, Hamood AN. The Pseudomonas aeruginosa exotoxin A regulatory gene, ptxS: evidence for negative autoregulation. J Bacteriol. 1999 Aug;181(16):4890-5. PubMed PMID: 10438759; PubMed Central PMCID: PMC93976.
• Rumbaugh KP, Hamood AN, Griswold JA. Analysis of Pseudomonas aeruginosa clinical isolates for possible variations within the virulence genes exotoxin A and exoenzyme S. J Surg Res. 1999 Mar;82(1):95-105. PubMed PMID: 10068532.
• Rumbaugh KP, Griswold JA, Hamood AN. Contribution of the regulatory gene lasR to the pathogenesis of Pseudomonas aeruginosa infection of burned mice. J Burn Care Rehabil. 1999 Jan-Feb;20(1 Pt 1):42-9. PubMed PMID: 9934636.
• Vasil ML, Ochsner UA, Johnson Z, Colmer JA, Hamood AN. The fur-regulated gene encoding the alternative sigma factor PvdS is required for iron-dependent expression of the LysR-type regulator ptxR in Pseudomonas aeruginosa. J Bacteriol. 1998 Dec;180(24):6784-8. PubMed PMID: 9852033; PubMed Central PMCID: PMC107792.
• Colmer JA, Hamood AN. Characterization of ptxS, a Pseudomonas aeruginosa gene which interferes with the effect of the exotoxin A positive regulatory gene, ptxR. Mol Gen Genet. 1998 May;258(3):250-9. PubMed PMID: 9645431.
• Colmer JA, Fralick JA, Hamood AN. Isolation and characterization of a putative multidrug resistance pump from Vibrio cholerae. Mol Microbiol. 1998 Jan;27(1):63-72. PubMed PMID: 9466256.
• Hamood AN, Griswold J, Colmer J. Characterization of elastase-deficient clinical isolates of Pseudomonas aeruginosa. Infect Immun. 1996 Aug;64(8):3154-60. PubMed PMID: 8757847; PubMed Central PMCID: PMC174201.
• Hamood AN, Colmer JA, Ochsner UA, Vasil ML. Isolation and characterization of a Pseudomonas aeruginosa gene, ptxR, which positively regulates exotoxin A production. Mol Microbiol. 1996 Jul;21(1):97-110. PubMed PMID: 8843437.
• Hamood AN, Griswold JA, Duhan CM. Production of extracellular virulence factors by Pseudomonas aeruginosa isolates obtained from tracheal, urinary tract, and wound infections. J Surg Res. 1996 Mar;61(2):425-32. PubMed PMID: 8656619.
• McVay CS, Hamood AN. Toxin A secretion in Pseudomonas aeruginosa: the role of the first 30 amino acids of the mature toxin. Mol Gen Genet. 1995 Dec 15;249(5):515-25. PubMed PMID: 8544817.
• Hamood AN, Griswold J. DNA hybridization analysis of the Pseudomonas aeruginosa elastase gene (lasB) from different clinical isolates. Can J Microbiol. 1995 Oct;41(10):910-7. PubMed PMID: 8590405.
• West SE, Kaye SA, Hamood AN, Iglewski BH. Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of exotoxin A. Infect Immun. 1994 Mar;62(3):897-903. PubMed PMID: 8112861; PubMed Central PMCID: PMC186200.
• Hamood AN, Ohman DE, West SE, Iglewski BH. Isolation and characterization of toxin A excretion-deficient mutants of Pseudomonas aeruginosa PAO1. Infect Immun. 1992 Feb;60(2):510-7. PubMed PMID: 1730483; PubMed Central PMCID: PMC257657.
• Olson JC, Hamood AN, Vincent TS, Beachey EH, Iglewski BH. Identification of functional epitopes of Pseudomonas aeruginosa exotoxin A using synthetic peptides and subclone products. Mol Immunol. 1990 Oct;27(10):981-93. PubMed PMID: 1700288.
• Hamood AN, Wick MJ, Iglewski BH. Secretion of toxin A from Pseudomonas aeruginosa PAO1, PAK, and PA103 by Escherichia coli. Infect Immun. 1990 May;58(5):1133-40. PubMed PMID: 2108926; PubMed Central PMCID: PMC258600.
• Wick MJ, Hamood AN, Iglewski BH. Analysis of the structure-function relationship of Pseudomonas aeruginosa exotoxin A. Mol Microbiol. 1990 Apr;4(4):527-35. Review. PubMed PMID: 2112672.
• Hamood AN, Iglewski BH. Expression of the Pseudomonas aeruginosa toxA positive regulatory gene (regA) in Escherichia coli. J Bacteriol. 1990 Feb;172(2):589-94. PubMed PMID: 2105298; PubMed Central PMCID: PMC208481.
• Hamood AN, Olson JC, Vincent TS, Iglewski BH. Regions of toxin A involved in toxin A excretion in Pseudomonas aeruginosa. J Bacteriol. 1989 Apr;171(4):1817-24. PubMed PMID: 2495264; PubMed Central PMCID: PMC209827.
• Hamood AN, Pettis GS, Parker CD, McIntosh MA. Isolation and characterization of the Vibrio cholerae recA gene. J Bacteriol. 1986 Jul;167(1):375-8. PubMed PMID: 3522553; PubMed Central PMCID: PMC212886.
• Hamood AN, Sublett RD, Parker CD. Plasmid-mediated changes in virulence of Vibrio cholerae. Infect Immun. 1986 May;52(2):476-83. PubMed PMID: 2939028; PubMed Central PMCID: PMC261023.

CONTACT INFO

• Email Address: abdul.hamood@ttuhsc.edu
• Contact Numbers
• Phone: 806.743.4057
• Lab: 806.743.1707
• Fax: 806.743.2334
• Physical Address: Department of Immunology and Molecular Microbiology, School of Medicine, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430