Biotechnology Applications By Brendan o'hara and john eble

What is PCR?

The Polymerase Chain Reaction is a method in Biology used to multiply certain strand(s) of DNA. It can make thousands or millions of exact copies.

How does PCR work?

PCR works by first heating up the DNA, so much so that the DNA unravels or denatures. When it denatures it splits into two pieces of single-stranded DNA. Ttyen an enzyme called Taq Polymerase builds new strands of DNA, modeling them after the originals. Each of these newly built strands of DNA are used to make two new copies and the process continues.

Provide one possible real world application for PCR.

Most mapping methods in the Human Genome Project use the Polymerase Chain Reaction.

What is gel electrophoresis?

In gel electrophoresis, proteins or molecules pass through an electrical field in a gel that contains small pores or openings. The molecules are then separated based on size and content. This technique is used to separate DNA, RNA or other proteins.

How does gel electrophoresis work?

Proteins or molecules pass through an electrical field in a gel that contains small pores or openings. The molecules are then separated based on size and content.

Provide one possible real world application for gel electrophoresis.

Some real world applications for gel electrophoresis are typing bacteria and tracking the sources and spread of disease.

What is DNA sequencing?

DNA sequencing is the process that makes the order of nucleotides in DNA. It is any method or technique that can determine the order of adenine, guanine, cytosine and thymine.

Provide one possible real world application for DNA sequencing.

One possible real world application for DNA sequencing was the revolution in medicine, the human genome project and genetics in forensic science.

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