Sarah Chapman is the point person for InVivo imaging, histology, sample preparation and image analysis and is working in close collaboration with her NDIIF counterparts within the Optical Microscopy Facility (Galvin building) and the Advanced Electron Microscopy Facility, in the Stinson-Remick building. This mode of operation is beneficial to the community as it provides extended support hours, wider expertise, and access to more equipment than each individual investigator can afford on their own. The EM branch of the facility is staffed by Alex Mukasyan (director and SEM expert) and Maksym Zhukovskyi (program director specializing in TEM). Tatyana Orlova is responsible for instrumentation training and scheduling. The OM branch of the facility is staffed by Sara Cole (director) and Sarah Chapman (serving as a technician). All faculty and staff report directly to Bradley Smith, who serves as an executive director under the management of Bob Bernhard. For administrative questions, contact Theresa Bollinger.
Mode of operation
Located within the basement floor of Galvin building, the InVivo Imaging and Optical Microscopy facility is accessible 24/7, and users can reserve time on each instrument by using an online calendar. The histology core is located on the ground floor of Galvin and is available to users throughout the working hours of M-F, 9-5 and by request on weekends. The Advanced Electron Microscopy facility located in the basement of Stinson-Remick is accessible 24/7 and is also available to users via an online calendar.
For light microscopy, sample preparation and image acquisition are typically handled by the end-users, with support and training provided by Sara Cole (imaging acquisition, data analysis, and specimen preparation). For electron microscopy (EM), most of the sample processing and imaging is done in-house by Tatyana Orlova to ensure consistent and reproducible conditions. Maksym Zhukovskyi is fully trained for sample processing and imaging on our TEM instruments. Sarah Chapman is fully trained for sample preparation of soft and biological materials for both SEM and TEM imaging.
After an initial orientation during which the project is discussed and the best approach is decided upon, users receive hands-on training on the equipment and/or software best suited to their goals, followed by continuous support when required. Additional training and support is offered to the community in different ways: (1) on-site assistance and training on equipment owned by individual investigators; (2) a yearly workshop on microscopy covering light and electron microscopy, image analysis, and sample processing, organized by the NDIIF.