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Detection Validation Studies and Applications Cyclospora Publications 

BAM Chapter 19b: Molecular Detection of Cyclospora cayetanensis in Fresh Produce Using Real-Time PCR

Resendiz-Nava CN, Orozco-Mosqueda GE, Mercado-Silva EM, Flores-Robles S, Silva-Rojas HV, Nava GM. A molecular tool for rapid detection and traceability of Cyclospora cayetanensis in fresh berries and berry farm soils. Foods, 2020, 9(3):261.

Assurian A, Murphy H, Ewing L, Cinar HN, Alexandre da Silva A, Almeria S. Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries. Food Microbiology, 2020, 87:103397.

Almeria S, da Silva AJ, Blessington T, Cloyd TC, Cinar HN, Durigan M, Murphy HR. Evaluation of the U.S. Food and Drug Administration validated method for detection of Cyclospora cayetanensis in high-risk fresh produce matrices and a method modification for a prepared dish. Food Microbiology, 2018, 76:497-503.

Gérard C, Franssen F, La Carbona S, Monteiro S, Cozma-Petruţ A, Utaaker KS, Jambrak AR, Rowan N, Rodríguez-Lazaro D, Nasser A, Tysnes K, Robertson LJ. Inactivation of parasite transmission stages: Efficacy of treatments on foods of non-animal origin. Trends in Food Science & Technology, 2019, 91:12-23.

Temesgen TT, Tysnes KR and Robertson LJ (2019) A New Protocol for Molecular Detection of Cyclospora cayetanensis as Contaminants of Berry Fruits. Front. Microbiol. 10:1939. doi: 10.3389/fmicb.2019.01939

Murphy HR, Cinar HN, Gopinath G, Noe KE, Chatman LD, Miranda NE, Wetherington JH, Neal-McKinney J, Pires GS, Sachs E, Stanya KJ, Johnson CL, Nascimento FS, Santin M, Molokin A, Samadpour M, Janagama H, Kahler A, Miller C, da Silva AJ. Interlaboratory validation of an improved method for detection of Cyclospora cayetanensis in produce using a real-time PCR assay. Food Microbiology, 2018, 69:170-178.

Murphy HR, Lee S, da Silva AJ. Evaluation of an improved U.S. Food and Drug Administration method for the detection of Cyclospora cayetanensis in produce using real-time PCR. Journal of Food Protection, 2017, 80:1133-1144. https://doi.org/10.4315/0362-028X.JFP-16-492