Heat plate: take precautions when using the heat plate so that you do not burn yourself
Eye Protection: you must wear goggles at all times while performing the experiment. This is so that chemicals do not end up getting into your eyes.
Touching the Solutions and Liquids: This should be avoided, especially with the denatured enzymes. The denatured where boiled so may be hot and burn your skin.
The purpose of this experiment was to test the glucose levels in the five solutions we mixed, milk and enzyme solution, milk and water, milk and denatured enzyme solution, sucrose solution and enzyme solution, and finally, sucrose solution and water.
Enzymes are a class of protein molecules that act as biological catalysts. These catalysts increase the rate of a reaction, but are not used up in the reaction. Enzymes are very similar to chemical catalysts but have many differences, as well. Enzymes are more specific than catalysts, meaning that they recognize particular substrates and convert them into products. The enzyme we studied was lactase, in a reaction this enzyme catalyzes the hydrolysis of the lactose into galactose and glucose. In simpler terms, lactase helps digest and break down lactose which is found in milk and other dairy products. When someone is lactose intolerant it means that the body produces a lower amount of lactase that is necessary to digest lactose. In “lactose-free” milk lactase enzymes are added into it to hydrolyze the lactose before drinking it.
Materials and Procedures
Lactase Tablet, 15 mL of Skim Milk, Water, Sucrose, 100 mL graduated cylinder, 10 mL graduated cylinder, 3 400 mL beakers, 5 test tubes, test tube rack, marking pencil, timer, hot plate with a pyrex test tube, Glucose test strip, stirring rod