A. Diagnosis of acute gonorrhea in males:
Specimen: urethral discharge, stained with Gram
The presence of Gram negative diplococci inside and outside pus cells is diagnostic.
B. Diagnosis of chronic male and acute and chronic female gonorrhea:
a. For males: morning drop, centrifuged urinary deposit or prostatic fluid after prostatic massage
b. For females: urethral and cervical discharge
2. Direct smear:
Stained with Gram. It is difficult to detect the organism either due to the presence of normal flora or due to the low number.
on chocolate agar or Modified Thayer Martin medium.
4. Recent methods for diagnosis:
- Direct fluorescent antibody test
- Antibody based particle agglutination
- DNA probes
- Direct detection of Gonococcal antigen in specimen by ELISA