Laboratory diagnosis of gonococcal infections I. Venereal gonorrhea

A. Diagnosis of acute gonorrhea in males:

Specimen: urethral discharge, stained with Gram

The presence of Gram negative diplococci inside and outside pus cells is diagnostic.

B. Diagnosis of chronic male and acute and chronic female gonorrhea:

1. Specimen:

a. For males: morning drop, centrifuged urinary deposit or prostatic fluid after prostatic massage

b. For females: urethral and cervical discharge

2. Direct smear:

Stained with Gram. It is difficult to detect the organism either due to the presence of normal flora or due to the low number.

3. Culture:

on chocolate agar or Modified Thayer Martin medium.

4. Recent methods for diagnosis:

  • Direct fluorescent antibody test
  • Antibody based particle agglutination
  • DNA probes
  • Direct detection of Gonococcal antigen in specimen by ELISA

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