Hypothesis and Variables
My hypothesis is that the DoTerra Oregano oil will be the most effective, and the hand sanitizer will be the least effective, but they will kill a substatial amount of the bacteria.
- Independent variables- The independent variables in this experiment would be the different disinfectants used, so the 3 DoTerra oils and hand sanitizer.
- Dependent variable- The variable that is changed and then measured based of the different independent variables, so in this experiment it would be the how much of the bacteria is killed.
1. First, I ordered all of the ingredients that I didn't have with me, which included the Petrie dishes, Agar Agar, Purell, and soap.
2. Then I filled the pot with 200ml of water and boiled it for 3 minutes to sterilize it. I let the water cool to just below boiling and added 4.5g of agar agar, and 2 tablespoons of beer stock. I stirred it until it dissolved and let it cool.
3. I added the cooled solution into the Petrie dishes so that they were a little less than half full, and put them in my fridge upside with the tops on to set overnight.
4. 24 hours later they were semi-solid gelatin so I began adding the bacteria. I wet a q-tip and lightly rubbed back and forth on my hand, and then lightly applied the bacteria from the q-tip onto the gelatin.
5. I did this for all 10 dishes, using a new q-tips each time, and then put the lids on.
6. I put the dishes on the tray and put them in my boiler room to culture, because the best condition is a warm environment. I let them grow for 4 days and then began the experiment.
7. I took the lids off of each dish, and wrote 2 of each name on the sticky notes. I put the sticky note on each lid so that there were 5 differently labeled dishes- Purell, soap, DoTerra Oregano, DoTerra Clove, and DoTerra Melissa.
8. After that, I took the measuring spoon and selected the 1/8 of teaspoon, which was the smallest one. I started with the hand sanitizer, so I filled the spoon about 3/4 full and put it on a dish labeled "purell".
9. I repeated this process, making sure I cleaned the spoon each time, until all of the 10 dishes had their 5 respective disinfectants.
10. I put the lids back on and waited 24 hours for the disinfectants to kick in.
11. Then I recorded the results of which one worked the best and killed the most bacteria.
ResuLts and analysis
Straight off the bat, the DoTerra Clove oil killed the most bacteria. I could tell that right away, but Purrel was a close second. The worst was DoTerra Melissa oil, but it wasn't that far behind. It still removed many of the white spots, which are the bacteria cultures. Another thing I noticed were some black spots, were not killed, or at least very little. The disinfectant soap and the DoTerra Oregano oil were very close, and it was difficult to tell because the spots were so small.